{
    "Identifier": "5821197A9EC384A02E6207D3A7C15ECF5971CB1C",
    "Last_Update": "2026-08-02T13:30:10",
    "Source": "ELIXIR-ENA",
    "Title": "Picoplankton 18S rRNA genes from the tropical and sub-tropical global-ocean sampled during the Malaspina-2010 expedition",
    "Abstract": "Surface global ocean eukaryotic picoplankton from the Malaspina-2010 expedition: Surface waters (3m depth) from a total of 122 globally-distributed stations located in the tropical and sub-tropical global ocean were sampled from December 2010 to July 2011 as a part of the Malaspina-2010 expedition (Duarte 2015 Estrada, et al. 2016). Water samples were obtained with Niskin bottles attached to a CTD profiler that included sensors for conductivity, temperature, oxygen, fluorescence and turbidity. About 12L of seawater were sequentially filtered through a 20\u00b5m nylon mesh, followed by a 3\u00b5m and 0.2\u00b5m polycarbonate filters of 142mm diameter (Isopore, Millipore). Only the picoplankton size-fraction (0.2-3 \u00b5m) was used for sequencing. DNA was extracted using a standard phenol-chloroform protocol (Massana, et al. 1997). The V4 region of the 18S (~380 bp) was amplified with the primers TAReuk454FWD1 and TAReukREV3 (Stoeck, et al. 2010). Duarte CM. 2015. Seafaring in the 21St Century: The Malaspina 2010 Circumnavigation Expedition. Limnology and Oceanography Bulletin 24:11-14.Estrada M, Delgado M, Blasco D, Latasa M, Cabello AM, Benitez-Barrios V, Fraile-Nuez E, Mozetic P, Vidal M. 2016. Phytoplankton across Tropical and Subtropical Regions of the Atlantic, Indian and Pacific Oceans. PLoS One 11:e0151699.Massana R, Murray AE, Preston CM, DeLong EF. 1997. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Applied and environmental microbiology 63:50-56.Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA. 2010. Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. Molecular ecology 19 Suppl 1:21-31.",
    "Keywords": [
        "ELIXIR-ENA"
    ],
    "Parameters": [
        "uncultured eukaryote"
    ],
    "Instruments": [
        "532"
    ],
    "Platforms": [
        "308"
    ],
    "Bounding_Box_WestLongitude": -179.524,
    "Bounding_Box_NorthLatitude": 35.134998,
    "Bounding_Box_EastLongitude": 179.14101,
    "Bounding_Box_SouthLatitude": -40.549999,
    "Temporal_Extent_Begin": "2010-12-16",
    "Temporal_Extent_End": "2011-07-10",
    "OnlineResourceUrl": "\/search-details?step=~0125821197A9EC384A02E6207D3A7C15ECF5971CB1C"
}