{
    "Identifier": "6B7BE1F172F86D9785F6A6E60E8EBBD2FC08F5D0",
    "Last_Update": "2026-07-12T14:18:26",
    "Source": "ELIXIR-ENA",
    "Title": "Alterations in the gut-associated microbiota of juvenile Caribbean spiny lobsters Panulirus argus (Latreille, 1804) infected with PaV1",
    "Abstract": "The spiny lobster Panulirus argus (Latreille, 1804) is currently affected by an unenveloped, icosahedral, DNA virus\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;termed Panulirus argus virus 1 (PaV1), a pathogenic and mortal virus that produces a long-lasting infection that alters\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;the physiology and behavior of heavily infected lobsters. In crustaceans, the gut-associated microbiota is crucial for\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;their homeostasis and well-being, but pathogens could change the composition of the gut-associated microbiota\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;affecting its function. In PaV1 infection, the changes of gut-associated microbiota are yet to be elucidated. In the\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;present study, we used high-throughput 16S rRNA sequencing technology to compare the bacterial microbiota of\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;intestines of healthy and heavily PaV1-infected male and female juveniles of P. argus captured in Puerto Morelos Reef\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;lagoon, Quintana Roo, Mexico. We found that basal gut-associated microbiota composition showed a sex dependent\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;bias, with females being enriched in Sphingomonas, while males were enriched in Candidatus Hepatoplasma and\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;Aliiroseovarius genera. Moreover, the alpha diversity of microbiota decreased in PaV1-infected lobsters. A significant\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;increase of Candidatus Bacilloplasma and Vibrio was observed in infected lobsters, as well as a significant decrease in\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;Nesterenkonia, Caldalkalibacillus, Vibrio, Pseudomonas, Cetobacterium and Phylobacterium. However, alterations in\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;the microbiota showed a sex-biased effect. Results from this study suggest that PaV1 infection impacts intestinal\u0026lt;\/p\u0026gt;\u0026lt;p\u0026gt;microbiota composition in P. argus in a sex-dependent manner.",
    "Keywords": [
        "ELIXIR-ENA"
    ],
    "Parameters": [
        "invertebrate gut metagenome"
    ],
    "Instruments": [
        "532"
    ],
    "Platforms": [
        "308"
    ],
    "Bounding_Box_WestLongitude": -86.519997,
    "Bounding_Box_NorthLatitude": 20.51,
    "Bounding_Box_EastLongitude": -86.519997,
    "Bounding_Box_SouthLatitude": 20.51,
    "Temporal_Extent_Begin": "2017-11-01",
    "Temporal_Extent_End": "2017-11-01",
    "OnlineResourceUrl": "\/search-details?step=~0126B7BE1F172F86D9785F6A6E60E8EBBD2FC08F5D0"
}